ac4C-acRIP-seq
RNA修饰介绍 | RNA修饰研究方法 | 嘉因生物RNA修饰服务优势
▍RNA修饰介绍
RNA上已经报道了超过170种RNA修饰，这些修饰影响着RNA的合成，转运，功能和代谢，进而调节细胞功能。近年来，包括m6A，m7G，ac4C，m5C等在内的RNA修饰成为生命科学的研究热点。
▍RNA修饰研究方法
RNA修饰的研究方法主要有两种，一是基于抗体的免疫共沉淀的IP法(RIP-Seq)，这个方法常用来研究m6A，m7G，ac4C等修饰，另外一种是基于化合物转换法比如亚硫酸氢盐的Bisulfite-seq，一般用来研究m5C和Ψ等修饰(后者也称为BID-seq)。
▍嘉因生物RNA修饰服务优势
优势一： 高分客户文章数量多。嘉因生物自2019年推出MeRIP-seq以来，协助客户发表文章100余篇，其中两篇m6A相关文献发表于顶刊Nature Genetics。
优势二： 高标准交付数据。
优势三： 实验硬件强。嘉因生物配备专门的冷室用于RNA的IP实验，防止RNA在操作中降解。此外我们使用TaKaRa微量建库试剂盒, m6A修饰最低只需1ug 的total RNA。
优势四： 高级分析经验丰富。各种分析应有尽有。
▍客户文章精选
文章标题 | 杂志 | 影响因子 |
Super-enhancer RNA m6A promotes local chromatin accessibility and oncogene transcription in pancreatic ductal adenocarcinoma | Nature Genetics | 41.376 |
RNA m6A regulates transcription via DNA demethylation and chromatin accessibility | Nature Genetics | 41.376 |
Insufficient Radiofrequency Ablation Promotes Hepatocellular Carcinoma Metastasis through m6A mRNA Methylation Dependent Mechanism | Hepatology | 14.971 |
m6A demethylase ALKBH5 drives denervation-induced muscle atrophy by targeting HDAC4 to activate FoxO3 signalling | Journal of Cachexia Sarcopenia and Muscle | 12.063 |
Downregulated METTL14 accumulates BPTF that reinforces super-enhancers and distal lung metastasis via glycolytic reprogramming in renal cell carcinoma | Theranostics | 11.6 |
PRMT5 regulates RNA m6A demethylation for doxorubicin sensitivity in breast cancer | Molecular Therapy | 11.454 |
m6A methylation regulates hypoxia-induced pancreatic cancer glycolytic metabolism through ALKBH5-HDAC4-HIF1α positive feedback loop | Oncogene | 8.756 |
ALKBH5-mediated m6A demethylation fuels cutaneous wound re-epithelialization by enhancing PELI2 mRNA stability | Inflammation and Regeneration | 8.1 |
N6-Methyladenosine METTL3 Modulates the Proliferation and Apoptosis of Lens Epithelial Cells in Diabetic Cataract | Molecular Therapy - Nucleic Acids | 7.032 |
METTL3 Facilitates Oral Squamous Cell Carcinoma Tumorigenesis by Enhancing c-Myc Stability via YTHDF1-Mediated m6A Modification | Molecular Therapy - Nucleic Acids | 7.032 |
RBM15 condensates modulate m6A modification of STYK1 to promote tumorigenesis | Computational and Structural Biotechnology Journal | 6.155 |
FTO promotes liver inflammation by suppressing m6A mRNA methylation of IL-17RA | Frontiers in Oncology | 5.738 |
Methylated RNA Immunoprecipitation Sequencing Reveals the m6 A Landscape in Oral Squamous Cell Carcinoma | Journal of Immunology Research | 4.493 |