▍CUT&Tag/scCUT&Tag介绍
CUT&Tag(Cleavage Under Targets & Tagmentation)能够检测特定转录因子的结合位点或特定组蛋白修饰的发生位点。自从2019年Steven Henikoff 在Nature Communications发布该技术以来(目前引用超过1000次),该技术以其相对简化的实验操作,相对宽松的抗体级别要求,逐渐代替了ChIP-seq(Chromatin Immunoprecipitation sequencing)技术,成为表观遗传学研究的核心技术之一。
CUT&Tag通过引导特定抗体和Tn5转座酶复合物到指定转录因子的结合位点/指定组蛋白修饰的发生位点上,从而实现对结合位点DNA的精准切割和标记。这些标记的DNA片段进一步通过PCR扩增和高通量测序,从而提供转录因子/组蛋白修饰在全基因组范围内的结合位点或发生位点信息。CUT&Tag技术在研究基因调控、转录因子结合、组蛋白修饰和染色质结构等方面具有广泛应用。
scCUT&Tag,即单细胞CUT&Tag,可以进一步了解,指定的转录因子或者组蛋白修饰,在细胞群体中每一个细胞中的结合位置。
▍ CUT&Tag vs. ChIP-seq
▍ 嘉因生物CUT&Tag的服务优势
优势一 2021年7月,国内第1篇由服务商提供CUT&Tag服务的文章发表于Genome Biology。截止至2023年8月,嘉因已累计协助客户发表CUT&Tag文章78篇,总影响因子超过600分,包括Nature Genetics, Signal Transduction and Targeted Therapy, Cell Discovery 等顶尖杂志。
优势二 嘉因生物8年来,积累了超过300种的成功实验抗体库,向老师们推荐高成功率的转录因子抗体和组蛋白修饰抗体。CUT&Tag项目进行高标准交付(人的经典DNA结合蛋白,MACS2 Q-value= 0.05下至少500个peak)。
优势三 延续嘉因生物5年来的ATAC-seq的丰富提核经验,针对各种组织器官类型的CUT&Tag项目的提核更得心应手。嘉因已完成1500+例CUT&Tag实验,涉及到的物种和组织器官组合200+。
优势四 生信分析售后团队可以完成各类CUT&Tag定制化分析。
▍ 客户CUT&Tag文章精选
项目 | 目标蛋白 | 样本类型 | 文章标题 |
CUT&Tag RNAseq | H3K4me1 H3K27ac | 子宫癌细胞C33a | TNPO1-mediated nuclear import of ARID1B promotes tumor growth in ARID1A-deficient gynecologic cancer |
CUT&Tag ChIP-seq | STAT3;CTCF | T细胞 | Multiomics Analysis Identifies SOCS1 as Restraining T Cell Activation and Preventing Graft-Versus-Host Disease |
CUT&Tag ATAC-seq | H3K27me3 | 花粉细胞核 | H3K27 methylation regulates the fate of two cell lineages in male gametophytes |
CUT&Tag &RNA-seq | H3K9me3 | 心肌脂肪间充质干细胞(ADSC) | Excessive branched-chain amino acid accumulation restricts mesenchymal stem cell-based therapy efficacy in myocardial infarction |
CUT&Tag | MBD2 | CD4+ T细胞 | MBD2 acts as a repressor to maintain the homeostasis of the Th1 program in type 1 diabetes by regulating the STAT1-IFN-γ axis |
CUT&Tag | H3K9me3 | 冰冻肝脏组织 | Telomere-to-telomere assembly of a fish Y chromosome reveals the origin of a young sex chromosome pair |
CUT&Tag | DUX4/IGH ,RAG1 | 细胞 | DNA crosslinking and recombination-activating genes 1/2(RAG1/2)are required for oncogenic splicing in acute lymphoblastic leukemia |
CUT&Tag | PPARα | 人急性早幼粒细胞细胞 NB4 | Interplay between hypertriglyceridemia and acute promyelocytic leukemia mediated by the cooperation of peroxisome proliferator-activated receptor alpha with the PML/RAR alpha fusion protein on super-enhancers |
CUT&Tag | EBF3 | 人鼻咽癌细胞HK1 | EBF3 reactivation by inhibiting the EGR1/EZH2/HDAC9 complex promotes metastasis via transcriptionally enhancing vimentin in nasopharyngeal carcinoma |
ATAC-seq CUT&Tag | METTL3 TET1 | 食管癌细胞 | RNA m6A regulates transcription via DNA demethylation and chromatin accessibility |
CUT&Tag | H3K4me3 | 冰冻鱼鳃组织 | H3K4me3 CUT&Tag and Transcriptome Analysis Reveal the Epigenetic Regulatory Landscape in Gill Tissue of Large Yellow Croaker (Larimichthys crocea) Under Low Salinity Stress |
CUT&Tag | YAP | 人膀胱平滑肌细胞 HBdSMC | YAP/Smad3 promotes pathological extracellular matrix microenviroment-induced bladder smooth muscle proliferation in bladder fibrosis progression |
CUT&Tag | H3K27ac | 肝脏组织 | Genome analysis reveals hepatic transcriptional reprogramming changes mediated by enhancers during chick embryonic development |
CUT&Tag | PD-L1;P- STAT3 | 人侵袭性脉络膜黑色素瘤细胞(Mum2b) | Nuclear PD-L1 promotes EGR1-mediated angiogenesis and accelerates tumorigenesis |
CUT&Tag | H3K9ac | 人类结肠癌细胞系(Caco2) | Elevated expression of histone deacetylase HDAC8 suppresses arginine-proline metabolism in necrotizing enterocolitis |
CUT&Tag | six1 | 肝癌细胞系 HepG2 | Detrimental role of SIX1 in hepatic lipogenesis and fibrosis of non-alcoholic fatty liver disease |